The study determined the level of conflict experienced by nurse supervisors, head nurses, and staff nurses and the effectiveness of conflict management by nurse managers in selected level III DOH hospitals in Metro Manila. The study used quantitative, descriptive, comparative, and correlational design. A researcher-made questionnaire was administered to 46 nurse supervisors, 143 head nurses, and 149 staff nurses selected through purposive sampling technique. The nurse supervisors experienced “Low Level” of conflict in the areas of communication, problem solving, role functions, and personality/individually-oriented matters. The head nurses experienced “Low Level” of conflict in their interaction with co-head nurses, staff nurses, and nurse supervisors. The staff nurses experienced “Low Level” of conflict in their interaction with heads. Effectiveness of the management of workplace conflict by the heads and collaborating was found to be “Always Effective.” Competing, compromising, and accommodating strategies were rated “Often Effective.” There was a significant difference in the level of conflict experienced by the nurse supervisors, head nurses, and staff nurses. Significant difference were found in nurse supervisors’ management of workplace conflict.